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CheckM is a tool for assessing the quality of metagenome-assembled genomes (MAGs) by estimating completeness and contamination using lineage-specific single-copy marker genes. It provides standardized metrics to determine if bins meet MIMAG quality standards (e.g., >90% complete, <5% contaminated). CheckM 1.x uses HMMER against a reference tree; CheckM2 (recommended) uses machine learning for faster, more accurate predictions across diverse taxa.
Lineage Placement: Assigns each bin to a taxonomic lineage based on marker gene content.
Marker Analysis: Identifies presence/copy number of lineage-specific single-copy markers.
Quality Estimation: Calculates completeness (% markers found) and contamination (% markers with >1 copy).
Output: Generates summary table, plots, and per-bin statistics.
Address: 800 Dong Chuan RD. Minhang District, Shanghai, China SJTU-Yale Joint Center for Biostatistics, SJTU
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